Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients

Simple item page
dc.contributor.authorPhillips, Richard Odame
dc.contributor.authorFrimpong, Michael
dc.contributor.authorAhor, Hubert Senanu
dc.contributor.authorSakyi, Samuel Asamoah
dc.contributor.authorAgbavor, Bernadette
dc.contributor.authoret. al
dc.date.accessioned2020-01-13T12:29:46Z
dc.date.accessioned2023-04-19T01:47:31Z
dc.date.available2020-01-13T12:29:46Z
dc.date.available2023-04-19T01:47:31Z
dc.date.issued2019-11-26
dc.descriptionAn article published by Diagnosticsen_US
dc.description.abstractIsothermal amplification techniques such as recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) for diagnosing Buruli ulcer, a necrotic skin disease caused by Mycobacterium ulcerans, have renewed hope for the molecular diagnosis of clinically suspected Buruli ulcer cases in endemic districts. If these techniques are applied at district-level hospitals or clinics, they will help facilitate early case detection with prompt treatment, thereby reducing disability and associated costs of disease management. The accuracy as well as the application of these molecular techniques at point of need is dependent on simple and fast DNA extraction. We have modified and tested a rapid extraction protocol for use with an already developed recombinase polymerase amplification assay. The entire procedure from “sample in, extraction and DNA amplification” was conducted in a mobile suitcase laboratory within 40 min. The DNA extraction procedure was performed within 15 min, with only two manipulation/pipetting steps needed. The diagnostic sensitivity and specificity of this extraction protocol together with M. ulcerans RPA in comparison with standard DNA extraction with real-time PCR was 87% (n = 26) and 100% (n = 13), respectively. We have established a simple, fast and e cient protocol for the extraction and detection of M. ulcerans DNA in clinical samples that is adaptable to field conditions.en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.citationDiagnostics 2019, 9, 204; doi:10.3390/diagnostics9040204en_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/11920
dc.language.isoenen_US
dc.publisherDiagnosticsen_US
dc.subjectMycobacterium ulceransen_US
dc.subjectPCRen_US
dc.subjectrecombinase polymerase amplificationen_US
dc.subjectpoint-of-careen_US
dc.titleRapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patientsen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients.pdf
Size:
1.18 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
license.txt
Size:
1.73 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed to upon submission
Description:
Download
Collections
Articles