Loop-Mediated Isothermal Amplification for Laboratory Confirmation of Buruli Ulcer Disease—Towards a Point-of-Care Test

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dc.contributor.authorBeissner, Marcus
dc.contributor.authorPhillips, Richard Odame
dc.contributor.authorBattke, Florian
dc.contributor.authorBauer, Malkin
dc.contributor.authorBadziklou, Kossi
dc.contributor.authoret. al
dc.date.accessioned2020-01-09T10:52:16Z
dc.date.accessioned2023-04-19T01:39:25Z
dc.date.available2020-01-09T10:52:16Z
dc.date.available2023-04-19T01:39:25Z
dc.date.issued2015-11-13
dc.descriptionAn article published by PLOS Neglected Tropical Diseasesen_US
dc.description.abstractAs the major burden of Buruli ulcer disease (BUD) occurs in remote rural areas, development of point-of-care (POC) tests is considered a research priority to bring diagnostic services closer to the patients. Loop-mediated isothermal amplification (LAMP), a simple, robust and cost-effective technology, has been selected as a promising POC test candidate. Three BUD-specific LAMP assays are available to date, but various technical challenges still hamper decentralized application. To overcome the requirement of cold-chains for transport and storage of reagents, the aim of this study was to establish a dry-reagentbased LAMP assay (DRB-LAMP) employing lyophilized reagents.en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.citationPLoS Negl Trop Dis 9(11): e0004219. doi:10.1371/journal.pntd.0004219en_US
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/11864
dc.language.isoenen_US
dc.publisherPLOS Neglected Tropical Diseasesen_US
dc.titleLoop-Mediated Isothermal Amplification for Laboratory Confirmation of Buruli Ulcer Disease—Towards a Point-of-Care Testen_US
dc.typeArticleen_US
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