Methoxy-flavones identified from Ageratum conyzoides induce capase -3 and -7 activations in Jurkat cells

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dc.contributor.authorAcheampong, Felix
dc.contributor.authorReilly, John
dc.contributor.authorLarbie, Christopher
dc.contributor.authorSpencer, Matthew
dc.contributor.authorGunderson, Karl
dc.contributor.authorAppiah-Opong, Regina
dc.contributor.authorHowson, Christopher
dc.contributor.authorPorier, Jennifer
dc.contributor.authorJoyce, Kellie
dc.contributor.authorJeliazkova, Valentina
dc.contributor.authorVoytek, Sarah
dc.contributor.authorGinsburg-Moraff, Carol
dc.contributor.authorThibodeaux, Stefan
dc.contributor.authorKublbeck, Jill
dc.contributor.authorAustin, Steven
dc.date.accessioned2020-09-28T15:40:44Z
dc.date.accessioned2023-04-19T02:16:56Z
dc.date.available2020-09-28T15:40:44Z
dc.date.available2023-04-19T02:16:56Z
dc.date.issued2017-10
dc.descriptionThis article is published in Journal of Medicinal Plants Research and also available at 10.5897/JMPR2017.6457en_US
dc.description.abstractNew therapies for leukemia are urgently needed due to adverse side effects, tumor resistance and lack of selectivity of many chemotherapeutic agents in clinical use. Ageratum conyzoides has been used in folklore medicine for managing leukemia and other cancers. Thus, this study aimed to investigate the effects of fractions, sub-fractions and purified compounds from the ethanol leaf extracts of A. conyzoides against Jurkat cells-model for acute T cell leukemia. A two-dimensional purification process using normal phase flash, followed by reverse phase purification was necessary to isolate pure methoxy-flavones, which were further characterized by Nuclear Magnetic Resonance (NMR) and MS-MS. The effect of fractions or pure compounds on cell viability was determined using either the MTT reagent or CellTiter-Blue® assay, while the caspase-3 and -7 activation was tested with Caspase-Glo® 3/7 assay. Prediction of compounds’ drug disposition profiles in vivo was measured with biomimetic affinity chromatography methodologies.en_US
dc.description.sponsorshipKNUSTen_US
dc.identifier.citationJournal of Medicinal Plants Research ,Vol. 11(38), pp. 583-590en_US
dc.identifier.uri10.5897/JMPR2017.6457
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/13115
dc.language.isoenen_US
dc.publisherJournal of Medicinal Plants Researchen_US
dc.subject: Ageratum conyzoidesen_US
dc.subjectmethoxy-flavonesen_US
dc.subjectJurkat, biomimetic affinity chromatographyen_US
dc.subjectcell viabilityen_US
dc.titleMethoxy-flavones identified from Ageratum conyzoides induce capase -3 and -7 activations in Jurkat cellsen_US
dc.typeArticleen_US
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