Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay
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Date
2019-02-01
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PLOS Neglected Tropical Diseases
Abstract
Access to an accurate diagnostic test for Buruli ulcer (BU) is a research priority according to
the World Health Organization. Nucleic acid amplification of insertion sequence IS2404 by
polymerase chain reaction (PCR) is the most sensitive and specific method to detect Mycobacterium
ulcerans (M. ulcerans), the causative agent of BU. However, PCR is not always
available in endemic communities in Africa due to its cost and technological sophistication.
Isothermal DNA amplification systems such as the recombinase polymerase amplification
(RPA) have emerged as a molecular diagnostic tool with similar accuracy to PCR but having
the advantage of amplifying a template DNA at a constant lower temperature in a shorter
time. The aim of this study was to develop RPA for the detection of M. ulcerans and evaluate
its use in Buruli ulcer disease.
Description
An article published by PLOS Neglected Tropical Diseases and available at doi.org/10.1371/journal.pntd.0007155
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Citation
PLoS Negl Trop Dis 13(2): e0007155. https://doi.org/10.1371/journal.pntd.0007155