Spectrum of antibiotic resistant bacteria and fungi isolated from chronically infected wounds in a rural district hospital in Ghana
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Date
2020
Journal Title
Journal ISSN
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Publisher
PLOS ONE
Abstract
Background
Chronic infected wounds are generally difficult to manage and treatment can be particularly
challenging in resource-limited settings where diagnostic testing is not readily available. In
this study, the epidemiology of microbial pathogens in chronically infected wounds in rural
Ghana was assessed to support therapeutic choices for physicians.
Methods
Culture-based bacterial diagnostics including antimicrobial resistance testing were per formed
on samples collected from patients with chronic wounds at a hospital in Asante Akim
North Municipality, Ghana. Fungal detection was performed by broad-range fungal PCR
and sequencing of amplicons.
Results
In total, 105 patients were enrolled in the study, from which 207 potential bacterial patho gens
were isolated. Enterobacteriaceae (n = 84, 41%) constituted the most frequently iso lated group
of pathogens. On species level, Pseudomonas aeruginosa (n = 50, 24%) and
Staphylococcus aureus (n = 28, 14%) were predominant. High resistance rates were docu mented,
comprising 29% methicillin resistance in S. aureus as well as resistance to 3rd gen eration
cephalosporins and fluoroquinolones in 33% and 58% of Enterobacteriaceae,
respectively. One P. aeruginosa strain with carbapenem resistance was identified. The
most frequently detected fungi were Candida tropicalis.
Description
This article is published by Plos One and is also available at https://doi.org/10.1371/
journal.pone.0237263
Keywords
Citation
Krumkamp R, Oppong K, Hogan B, Strauss R, Frickmann H, Wiafe-Akenten C, et al. (2020) Spectrum of antibiotic resistant bacteria and fungi isolated from chronically infected wounds in a rural district hospital in Ghana