Unequal distribution of resistance-conferring mutations among Mycobacterium tuberculosis and Mycobacterium africanum strains from Ghana

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dc.contributor.authorOsei Ivy
dc.contributor.authorOwusu-Dabo Ellis
dc.contributor.authorChinbuah Amanua
dc.contributor.authorGyapong John
dc.contributor.authorNiemann Stefan.....et al
dc.date.accessioned2023-12-05T16:16:44Z
dc.date.available2023-12-05T16:16:44Z
dc.date.issued2010
dc.descriptionThis article is published by Elsevier and is also available at www.elsevier.de/ijmm
dc.description.abstractIsoniazid (INH) and rifampicin (RMP) resistance in Mycobacterium tuberculosis complex (MTC) isolates are mainly based on mutations in a limited number of genes. However, mutation frequencies vary in different mycobacterial populations. In this work, we analyzed the distribution of resistance-associated mutations in M. tuberculosis and M. africanum strains from Ghana, West Africa. The distribution of mutations in katG, fabG1-inhA, ahpC, and rpoB was determined by DNA sequencing in 217 INH-resistant (INHr ) and 45 multidrug-resistant (MDR) MTC strains isolated in Ghana from 2001 to 2004. A total of 247 out of 262 strains investigated (94.3%) carried a mutation in katG (72.5%), fabG1-inhA (25.1%), or ahpC (6.5%), respectively. M. tuberculosis strains mainly had katG 315 mutations (80.1%), whereas this proportion was significantly lower in M. africanum West-African 1 (WA1) strains (43.1%; p < 0.05). In contrast, WA1 strains showed more mutations in the fabG1-inhA region (39.2%, p < 0.05) compared to M. tuberculosis strains (20.9%). In 44 of 45 MDR strains (97.8%) mutations in the 81-bp core region of the rpoB gene could be verified. Additionally, DNA sequencing revealed that 5 RMP-susceptible strains also showed mutations in the rpoB hotspot region. In conclusion, although principally the same genes were affected in INHr M. tuberculosis and M. africanum strains, disequilibrium in the distribution of mutations conferring resistance was verified that might influence the efficiency of molecular tests for determination of resistance
dc.description.sponsorshipKNUST
dc.identifier.citationInternational Journal of Medical Microbiology 300 (2010) 489–495
dc.identifier.uri10.1016/j.ijmm.2010.04.019
dc.identifier.urihttps://ir.knust.edu.gh/handle/123456789/14626
dc.language.isoen
dc.publisherELSEVIER
dc.titleUnequal distribution of resistance-conferring mutations among Mycobacterium tuberculosis and Mycobacterium africanum strains from Ghana
dc.typeArticle
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