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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/9054

Title: Phytochemical screening and bioactive properties of the stem bark extracts of Allanblackia Parviflora (Chevalier) (Clusiaceae)
Authors: Amoah, Eunice
Issue Date: 3-Oct-2016
Abstract: The phytochemical constituents, antioxidants, antimicrobial and anti-inflammatory activities of the methanolic, aqueous and pet- ether extracts of the stem bark of Allanblackia parviflora have been studied in this work with the aim of exploring the medicinal properties of the plant in Ghana. The results obtained from the qualitative phytochemical screening indicated the presence of alkaloids, tanins, flavonoids, cardiac glycosides, reducing sugar, triterpenoids, anthraquinones, saponins and phytosterols and the absence of cyanogenic glycosides. The antimicrobial assay employed Agar-well diffusion and Micro broth dilution methods against 10 microbial strains (i.e. one fungus, five gram-negative and four gram-positive bacteria) using Ciprofloxacin as the reference drug. The methanolic extract exhibited the highest activity against E. faecalis with zone of inhibition 20 mm and MIC of 2.5 mg/ml. Pet-ether extract on the other hand was inactive against test microbes. Anti-inflammatory properties of the extracts employed the Carrageenan induced paw model in a week old chicks. The various extracts at 30, 100 and 300 mg/kg exhibited prominent therapeutic anti-inflammatory properties with methanol recording the highest activity at oedema inhibitory percentages of (42.92%, 56.93% and 66.01%) and (49.26%, 51.41% and 59.05%) for therapeutic and prophylatic studies respectively. Followed by aqueous (28.61%, 46.60% and 56.93%), (29.27%, 39.20% and 48.24%) and pet-ether (34.92%, 45.38% and 45.99%), (33.42%, 36.05% and 42.53%) respecitively. Antioxidant assay also employed DPPH scavenging potential, TPC and TAC for the assessment. The results however presented the methanolic extract as most potent with EC50 value of 0.03341 mg/ml, TAE of 212.85±0.311 mg/g and AAE of 77.72±0.549 mg/g for DPPH Scavenging potential, TPC and TAC respectively. These results therefore provide for the first time scientific bases for the folkloric use of A.parviflora in managing inflammations, microbial infections and some tropical diseases.
Description: Dissertation submitted to the Department of Pharmaceutical Chemistry of the Faculty of Pharmacy and Pharmaceutical Sciences KNUST, in partial fulfilment of the requirements for the award of Master of Philosophy Degree in Pharmaceutical Chemistry, 2016
URI: http://hdl.handle.net/123456789/9054
Appears in Collections:College of Health Sciences

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