Characterisation of Jasmine 85 rice (Oryza sativa) variety from different sources of seed production in Ghana
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Date
2014-10-20
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Abstract
Following the observation that rice variety Jasmine 85 from two sources in Ghana were different
in morphology, samples of Jasmine 85 were collected from seven sources in Ghana. A reference
source was obtained from Africa Rice Centre in Senegal for comparison. An experiment was set
up in a Randomized Complete Block Design and under standard conditions in Nobewam
(Ashanti region) to ascertain if there were any differences among the sources. Morphological
data was taken in the field to identify off types and characterise the sources. All the sources were
not significantly different in terms of aroma, anthocyanin coloration, leaf pubescence, and ligule
shape, but the sources showed significant differences with regards to pericarp colour, days to
50% heading, plant height, number of tillers, seed length, seed width, panicle length, and number
of secondary branches. Physico-chemical analyses were done for further characterisation. Grain
size and shape, grain chalkiness, cooking time, head rice yield, gelatinisation temperature,
amylose content, and viscosity properties were significantly different among the sources. Grain
hardness was not significantly different among the sources. Molecular characterisation using 15
SSR markers was done to establish the genetic resemblance among the sources. The sources
differed significantly, although the result also showed that they are closely related. A cluster
analysis run on the morphological and physico-chemical data gave four clusters: (GBEWAA and
TONO), (SARI), (PV), (KIP, CRI, ARI, DARTEY). The molecular data on the other hand gave
three clusters: (TONO, DARTEY, PV), (SARI, ARI, CRI), (KIP, GBEWAA). The results imply
that seeds from different sources should not be mixed for production, and these varieties should
be treated separately in future evaluations.
Description
A thesis submitted to the School of Research and
Graduate Studies, Kwame Nkrumah University of Science
and Technology, in partial fulfillment of the
requrememts for the award of Master of Philosophy
(Seed Science and Technology) degree.