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Title: | Prevalence and Antifungal Susceptibility of Candida Species Isolated from Women Attending A Gynaecological Clinic in Kumasi, Ghana |
Authors: | Abruquah, H. H. |
Keywords: | Antifungal susceptibility Candida species yeasts infections Fluconazole vulvovaginitis |
Issue Date: | Aug-2012 |
Publisher: | Journal of Science and Technology |
Citation: | Journal of Science and Technology, Vol. 32, No. 2, 2012, pp 39-45 |
Abstract: | Candidal vulvovaginitis causes extreme discomfort and affects the well being of women. The aim
of this study was to determine the prevalence of Candida infections among women attending
gynaecological clinic at the Komfo Anokye Teaching Hospital (KATH) in Kumasi and the antifungal
susceptibility patterns of the Candida species isolated. A total of 186 high vaginal swabs
(HVS) were collected from women suspected of having candidal vulvovaginitis between March
and June 2009. Wet mount preparations in potassium hydroxide (KOH) and Gram-stained
smears were performed directly on specimens. Cultures for Candida species were done using
sabouraud dextrose agar (SDA) (Oxoid, UK) at room temperature (25 - 28ºC). Antifungal susceptibility
of the Candida species to amphotericin B, fluconazole and itraconazole was assessed
using the ATB FUNGUS 3 test kits (bioMerieux, Marcy-l’Etoile, France). Of the 186 HVS cultured,
39 yielded Candida species giving Candida prevalence in HVS during the study period as
21%. Candida species isolated from HVS specimens were Candida albicans (n=19, 48.7%), Candida
glabrata (n=7, 17.9%), Candida tropicalis (n=4, 10.3%) and Candida dubliniensis (n=4,
10.3%). Other species isolated were Candida sake (n=2, 5.1%), Candida krusei (n=2, 5.1%) and
Candida parapsilosis (n=1, 2.6%). Susceptibility of the isolates to antifungal agents ranged from
66.7% to 87.2% with MICs ranging from ≤ 0.125mg/l to 8mg/l. Amphotericin B was the best performing
antifungal agents with sensitivity of 87.2%. |
Description: | Article published in the Journal of Science and Technology, Vol. 32, No. 2, 2012, pp 39-45 |
URI: | http://hdl.handle.net/123456789/5606 |
Appears in Collections: | Journal of Science and Technology 2000-
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