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Title: | Development and Evaluation of Lyophilized Thiolated-Chitosan Wafers for Buccal Delivery of Protein |
Authors: | Ayensu, I. Boateng, J. S. |
Keywords: | Thiolated-chitosan freeze-drying annealing buccal delivery protein |
Issue Date: | Aug-2012 |
Publisher: | Journal of Science and Technology |
Citation: | Journal of Science and Technology, Vol. 32, No. 2, 2012, pp 46-55 |
Abstract: | Annealed and non-annealed freeze-dried wafers from synthesised thiolated-chitosan have been
developed and evaluated. Wafers were obtained by freeze-drying aqueous gels of the thiolated
polymer incorporating per polymer weight, 10 % each of glycerol as plasticizer, D-mannitol as
cryoprotectant and 50% BSA as model protein drug. The formulation was freeze-dried with or
without the process of annealing. Texture analyzer was employed to investigate the in vitro mucoadhesive
properties in tensile mode, residual moisture content by thermo-gravimetric analysis
(TGA) while hydration capacity and drug release studies were performed in 0.1M PBS. Microscopic
architecture was examined using scanning electron microscopy (SEM). Differential scanning
calorimetry (DSC) was employed for protein phase separation studies and conformational
stability by attenuated total reflectance-Fourier transform infra-red spectroscopy (ATR-FTIR)
and confirmed with Circular dichroism (CD). The annealing process led to wafers with increased
ease of hydration, improved in vitro mucoadhesive characteristics and enhanced BSA
release, without affecting the conformational stability of the protein due to the presence of a
cryoprotectant. These results show the potential application of annealed freeze-dried thiolatedchitosan
wafers for buccal mucosa delivery of protein based drugs. |
Description: | Article published in the Journal of Science and Technology, Vol. 32, No. 2, 2012, pp 46-55 |
URI: | http://hdl.handle.net/123456789/5602 |
Appears in Collections: | Journal of Science and Technology 2000-
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