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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/5168

Title: Simultaneous Determination of Sulphamethoxazole and Trimethoprim in Co-Trimoxazole Tablets using Rp-Lc Analysis
Authors: Okine, N. N. A
Asare-Nkansah, S.
Acheampong, A.
Keywords: Septrin
Co-trimoxazole
Sulphamethoxazole
Trimethoprim
Salicylic Acid
Rp-lc
Internal Standard
Issue Date: Dec-2006
Publisher: Journal of Science and Technology
Citation: Journal of Science and Technology, Vol. 26 No. 3, 2006 pp 66-74
Abstract: A reversed phase liquid chromatographic (rp-lc) method has been developed to determine simultaneously the amount of sulphamethoxazole and trimethoprim in co-trimoxazole tablets using salicylic acid as internal standard. The chromatographic resolution nuts achieved with 96% ethanol and 0. IM ammonium perchlorate buffer (95:5) on a reversed phase column at ambient temperature. VV detection was at 254 nm. The flow through the column was 0.8ml/min with a chart recorder speed of 5mm/min. Mean retention times for trimethoprim sulphamethoxazole, and salicylic acid were 3.4 ± 0.13 min, 3.8 ± 0.08min, and 4.5 ± 0.13 min respectively. Calibration curves were linear over the ranges 0.0001-0.0006% w/v (sulphamethoxazole) and 0.002-0.012% w/v (Trimethoprim). The method has been used to analyze a branded co-trimoxazole (Septrin) tablets and a locally formulated co-trimoxazole tablets. The percentage content of sulphamethoxazole and trimethoprim determined by the rp-lc and the official u.v methods were found to be comparable and within the BP 2000 standards. Comparing this work with that of Ronn et al 1999, revealed that, this method is cost effective for routine analytical work as the mobile phase is devoid of ace-tonitrile, an expensive reagent. Additionally, running time for the current method (4 min) is shorter than that of Ronn et al (7 min), for in-process and finished product quality monitoring.
Description: Article published in the Journal of Science and Technology, Vol. 26 No. 3, 2006 pp 66-74
URI: http://hdl.handle.net/123456789/5168
Appears in Collections:Journal of Science and Technology 2000-

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