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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/5071

Title: Effect of Carbon Source (Sucrose) on the Morphogenesis of Pineapple (Ananas Comosus (L) Merr) Cultured In-Vitro
Authors: Adjei, P.Y.
Keywords: Photosynthetic Photon Flux (PPF)
Tissue Culture
Skoog and Murashige (MS)
Issue Date: 2001
Publisher: Journal of Science and Technology
Citation: Journal of Science and Technology, Vol. 21 Nos 1,2 & 3 2001. pp 29-32
Abstract: Sucrose is used in tissue culture as a source of carbon which is normally fixed by plants from the air. Some plants can make use of the physical environment and fix carbon (autotrophy) in tissue culture but such growth have been reported to be relatively slow. The level of sucrose in tissue culture can affect the growth and development of the plant. The type of specie of plant may react differently to the sucrose levels. The paper reports of different levels of sucrose and the effect of activated charcoal in the culture medium. The sucrose concentration significantly affected the number of shoots regenerated. The mean shoots number regenerated at 3% level (8.20) was significantly different from either the 2. 1 or no – sucrose medium. The extension of the original shoots was highest at the 3% level and lowest when no-sucrose was present by about 99.2% than the no-sucrose medium and 61.7% than the 2% medium. Activated charcoal (AC) alone in the medium did not induce morphogenesis/organogenesis significantly in the absence of sucrose (in pineapple) but affected plant growth.
Description: Article published in the Journal of Science and Technology, Vol. 21 Nos 1,2 & 3 2001. pp 29-32
URI: http://hdl.handle.net/123456789/5071
Appears in Collections:Journal of Science and Technology 2000-

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