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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/2996

Title: In-vitro regeneration of explants of Keitt, Jaffna and local varieties of Mango (Mangifera indica L.)
Authors: Ousman, Dauda Jarju
Issue Date: 19-Feb-1998
Series/Report no.: 2507;
Abstract: One of the major problems in most mango producing countries in the West African sub-region is inadequate supply of healthy planting materials of desirable cultivars. There is therefore, the need to determine the most appropriate explant and medium for in-vitro mass propagation of mango planting materials of desirable cultivars. The research conducted at Crops Research Institute, Kumasi, Ghana, was to determine the most appropriate explant and most suitable medium for mass propagation of mango planting materials. The MS medium with various concentrations and combinations of BA, KIN and GA3 supplemented with sucrose and PVP were used to culture flower, shoot tip, apical meristem and nodal bud explants. There was a high percentage (7 5-95 %) of contamination. This may have caused the failure of the explants to regenerate. The decontamination techniques of using 30 % sodium hypochlorite for 30 minutes instead of 20 % sodium hypochlorite for 20 minutes currently used for other crops, and spraying experimental branches with mixture of fungicide (Benlate 0.1 %) and antibiotic (Streptomycin 0.1%) before inoculation did not completely decontaminate the explants in-vitro, but it reduced the percentage of contamination. The B5 medium with various concentrations and combinations of BA, GA3 and KIN supplemented with NAA, KIN, 2, 4- D, sucrose and PVP were used to culture embryo explants. Calli and plantlets were generated from embryo of mature polyembryonic fruits of a Local variety and monoembryonic fruits of Keitt variety. The well-established plantlets in-vitro were transplanted into polythene bags containing soil medium in the screen house where they have established and are developing satisfactorily.
Description: A thesis submitted to the Board of Postgraduate Studies, Kwame Nkrumah University of Science and Technology, Kumasi, in partial fulfilment of the requirement for the award of the Degree of Master of Science in in Pomology, 1998
URI: http://hdl.handle.net/123456789/2996
Appears in Collections:College of Agric and Natural Resources

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