The genetics of maturity and its correlated traits and stability analysis in maize (zea mays l.) (a) identification of genomic regions controlling maturity and its correlated traits in b73 x unadapted mexican maize population. (b) evaluation of genotype by environment interactions in maize.
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Date
2001-12-13
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Abstract
This study was conducted to (i) apply biotechnological approaches. viz simple sequence repeats (SSR) primers and restriction fragment length polymorphism (RFLP) probes with classical breeding procedures to identify genes important in adapting exotic maize germplasm, and (ii) understand the mode of inheritance of maturity in adapted by unadapted F2 maize population. Two hundred and eleven (211) S1 families developed from a cross between adapted Corn Belt maize inbred line (B73) and an unadapted maize population and their testcrosses were evaluated at two planting dates and two locations, respectively, in 1995. Maturity was quantitatively inherited, and few factors were responsible for its expression in maize. Earliness was dominant to lateness. Fingerprinted DNA isolates from each family with SSR primers and RFLP probes revealed that, putative quantitative trait loci associated with maturity were located on seven of the ten chromosomes. Phi085, Phi 113, Phi014, Pigmentation umc59, umc168 and bnl5.09 were associated with maturity. The gene effects of the markers were a combination of additive and dominance. Ear and plant height were also quantitatively inherited and were
linked to Pigmentations umc59, umc34, csu5, phi014, bnl5.09 and bnl7.49. Pigmentation, csu25, phi0l4, bnl7.49 and umcl09 were also associated days to anthesis, grain moisture at harvest and grain yield in testcrosses. The use of molecular markers in conjunction with classical breeding methods was an efficient approach to understanding the mode of inheritance of maturity in maize.
Description
A thesis submitted to the Department of Crop Science, College of Agriculture and Natural Resources,
Kwame Nkrumah University of Science and Technology, in partial
fulfilment of the requirements for the award of Doctor of Philosophy degree in Plant Breeding and Genetics, 2001