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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/15678

Title: blaCTX-M-15 carried by IncF-type plasmids is the dominant ESBL gene in Escherichia coli and Klebsiella pneumoniae at a hospital in Ghana
Authors: Agbenyega, Edward Tsiri
Agyekum, Alex
Fajardo-Lubián, Alicia
Ansong, Daniel
Partridge, Sally R.
Iredell, Jonathan R.
Keywords: PFGE
Antibiotic resistance
CTX-M enzymes
Issue Date: 9-Sep-2015
Publisher: Elsevier Ltd
Citation: Elsevier Ltd, 2015
Abstract: Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases (ESBLs) are among the most multidrug-resistant pathogens in hospitals and are spreading worldwide. Horizontal gene transfer and spread of high-risk clones are involved in ESBL dissemination. Investigation of the resistance phenotypes of 101 consecutive clinical E. coli (n=58) and K. pneumoniae (n=43) isolated at the Komfo Anokye Teaching Hospital inGhana over 3months revealed 63 (62%)with an ESBL phenotype. All 63 had a blaCTX-Mgene, and sequence analysis showed that 62 of thesewere blaCTX-M-15. blaCTX-M-15was linked to ISEcp1 and orf477Δ in all isolates, and most isolates also carried blaTEM, aac(3)-II, aacA4cr, and/or blaOXA-30 genes on IncF plasmids. XbaI/pulsed-field electrophoresis showed heterogeneity among isolates of both species, suggesting that blaCTX-M-15 dissemination is caused by horizontal gene transfer rather than clonal spread of these species in Ghana.
Description: This article is published at Elsevier Ltd, and also available at http://dx.doi.org/10.1016/j.diagmicrobio.2015.12.010
URI: http://dx.doi.org/10.1016/j.diagmicrobio.2015.12.010
Appears in Collections:College of Health Sciences

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