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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/14425

Title: A ten-year (2007-2016) review of the prevalence of human papillomavirus in head and neck cancers at Komfo Anokye Teaching Hospital
Authors: Aboagye, Evans
Yeboah, F. A.
Keywords: Prevalence of Human Papillomavirus
Head and Neck Cancers
Komfo Anokye Teaching Hospital
Issue Date: 16-Jul-2021
Abstract: BACKGROUND Fewer studies have been done over the years to establish the association of human papillomavirus (HPV) with head and neck squamous cell carcinoma (HNSSC) within the subregions of sub-Saharan Africa, and thus this study was designed to investigate the presence of HPV DNA in HNSCC cases at Komfo Anokye Teaching hospital in Ghana, providing additional evidence on the need to explore similar studies in other subregions. STUDY SETTING The study was retrospective cross-sectional in design in which laboratory analysis was employed to investigate the presence of the DNA of HPV genotypes in HNSCC archived tissue samples obtained from the Department of Pathology, KATH, from January 2007 to December 2016. METHOD Formalin-fixed paraffin embedded (FFPE) tissue blocks and slides of selected HNSCC cases were retrieved consecutively. Genomic DNA was then prepared from 10µm FFPE tissue section using the Quick-DNATM FFPE kit. A nested multiplex PCR assay that combines degenerate E6/E7 consensus primers and type-specific primers was utilized for the detection of HPV genotypes (16, 18, 31, 33, 35, 45, 51, 52, 56, 58, 59, 6/11, 42, 43 and 44) in three cocktails. RESULTS A total of 301 HNSCC cases were extracted from the surgical day book, with male preponderance of 72.1% and a mean age of 54.97 years. The most common site of occurrence was the oral cavity (28.9%), followed by larynx (26.2%). Majority of the samples of patients aged <30 years were poorly differentiated (65.7%). HPV E6/E7 oncogenic DNA was detected in 18% of all HNSCC cases tested (n=100), with 17 been HPV-16 and 1 dual infection with HPV- 16 and HPV-18. A higher prevalence of HPV DNA was found in males (83.3%), and there was a trend towards patients older than 56 years likely to have HPV tumours (OR=1.378). HPV was prevalent in 50% of oropharyngeal cancers, 27% of laryngeal cancers, and 23% of oral cavity cancers. CONCLUSION HPV E6/E7 oncogenic DNA was found in 18% of HNSCC cases tested, with HPV-16 been the predominant genotype present. The pattern of HPV association was similar to earlier reported studies, recording a higher prevalence in oropharyngeal cancers, followed by laryngeal and oral cavity cancers
Description: A thesis submitted to the Department of Molecular Medicine, Kwame Nkrumah University of Science and Technology, Kumasi in partial fulfillment of the requirements for the Award of Degree in Master of Philosophy in Chemical Pathology. May, 2019
URI: http://hdl.handle.net/123456789/14425
Appears in Collections:College of Health Sciences

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