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|Title: ||Clinical and Laboratory Diagnosis of Buruli Ulcer Disease: A Systematic Review|
|Authors: ||Sakyi, Samuel Asamoah|
Aboagye, Samuel Y.
Otchere, Isaac Darko
|Issue Date: ||2016|
|Publisher: ||Canadian Journal of Infectious Diseases and Medical Microbiology|
|Citation: ||Canadian Journal of Infectious Diseases and Medical Microbiology, Volume 2016, Article ID 5310718, 10 pages, http://dx.doi.org/10.1155/2016/5310718|
|Abstract: ||Background. Buruli ulcer (BU) is a necrotizing cutaneous infection caused byMycobacterium ulcerans. Early diagnosis is crucial to
preventmorbid effects and misuse of drugs.We review developments in laboratory diagnosis of BU, discuss limitations of available
diagnostic methods, and give a perspective on the potential of using aptamers as point-of-care. Methods. Information for this
review was searched through PubMed, web of knowledge, and identified data up to December 2015. References from relevant
articles and reports from WHO AnnualMeeting of the Global Buruli Ulcer initiative were also used. Finally, 59 articles were used.
Results. The main laboratory methods for BU diagnosis are microscopy, culture, PCR, and histopathology. Microscopy and PCR
are used routinely for diagnosis. PCR targeting IS2404 is the gold standard for laboratory confirmation. Culture remains the only
method that detects viable bacilli, used for diagnosing relapse and accrued isolates for epidemiological investigation as well as
monitoring drug resistance. Laboratory confirmation is done at centers distant from endemic communities reducing confirmation
to a quality assurance. Conclusions.Current efforts aimed at developing point-of-care diagnostics are saddledwithmajor drawbacks;
we, however, postulate that selection of aptamers against MU target can be used as point of care.|
|Description: ||An article published in Canadian Journal of Infectious Diseases and Medical Microbiology, Volume 2016, Article ID 5310718, 10 pages, http://dx.doi.org/10.1155/2016/5310718|
|Appears in Collections:||College of Health Sciences|
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