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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/13591

Title: Fetal RhesusDGenotyping and Sex Determination from Maternal Plasma of Rhesus D-Negative Antenatal Population: The Usefulness of Conventional Polymerase Chain Reaction in Resource-limited Settings
Authors: Addai-Mensah, Otchere
Afriyie, Edward Y.
Sakyi, Samuel Asamoah
Obirikorang, Christian
Annani-Akollor, Max Efui
Owiredu, Eddie-Williams
Amponsah, Francis A.
Duneeh, Richard Vikpebah
Adu, Evans Asamoah
Issue Date: 2020
Publisher: Obstetrics and Gynecology International
Citation: Obstetrics and Gynecology International,Volume 2020, Article ID 4913793, 6 pages, https://doi.org/10.1155/2020/4913793
Abstract: Background. +is prospective cohort study evaluated the usefulness of conventional PCR in genotyping fetal Rhesus D (RhD) and sex from the maternal plasma of RhD-negative (RhD− ) antenatal population in resource-limited settings. Methods. +irty apparently healthy RhD− pregnant women with RhD positive (RhD+) partners were included. Blood samples were collected from each participant (in the third trimester of pregnancy) for DNA extraction/purification and fetal RhD genotyping. Results. Out of the 30 samples, 26 (86.7%) were found to be RhD+ while 4 (13.3%) were RhD− . +e RhD+ comprised 24 (80.0%) RhD+ based on exons 5, 7, and 10 combined. Exons 5 and 7 were detected in two additional samples but not exon 10. Serological phenotyping of neonatal blood confirmed 26 RhD+ and 4 RhD− . +ere was a perfect agreement between the fetal RhD genotype and neonatal RhD phenotyping after delivery for exons 5 and 7 (concordance = 100%, κ = 100.0%, diagnostic accuracy = 100%, p < 0.0001) while exon 10 presented with an almost perfect agreement (concordance = 93.3%, κ = 76.2%, diagnostic accuracy = 93.3%, p < 0.0001). Regarding the prenatal test for the SRY gene, 9 (30.0%) were predicted to be males and the remaining 21 (60.0%) were females. All the 9 and 21 anticipated males and females, respectively, were confirmed after delivery (concordance = 100%, κ = 100.0%, diagnostic accuracy = 100%). Conclusion. Our study suggests that conventional PCR using the SRY, RhD exons 5 and 7 could be useful for predicting fetal sex and RhD from maternal peripheral blood in resource-limited settings.
Description: An article published in Obstetrics and Gynecology International,Volume 2020, Article ID 4913793, 6 pages, https://doi.org/10.1155/2020/4913793
URI: http://hdl.handle.net/123456789/13591
Appears in Collections:College of Health Sciences

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