DSpace
 

KNUSTSpace >
Research Articles >
College of Health Sciences >

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/13412

Title: CD25 FoxP3 Memory CD4 T Cells Are Frequent Targets of HIV Infection In Vivo
Authors: Chachage, Mkunde
Pollakis, Georgios
Osei Kuffour, Edmund
Haase, Kerstin
Bauer, Asli
Nadai, Yuka
Podola, Lilli
Clowes, Petra
Schiemann, Matthias
Henkel, Lynette
Hoffmann, Dieter
Joseph, Sarah
Bhuju, Sabin
Maboko, Leonard
Sarfo, Fred Stephen...et.al
Issue Date: 2016
Publisher: Journal of Virology
Citation: Chachage M, Pollakis G, Kuffour EO, Haase K, Bauer A, Nadai Y, Podola L, Clowes P, Schiemann M, Henkel L, Hoffmann D, Joseph S, Bhuju S, Maboko L, Sarfo FS, Eberhardt K, Hoelscher M, Feldt T, Saathoff E, Geldmacher C. 2016. CD25 FoxP3 memory CD4 T cells are frequent targets of HIV infection in vivo. J Virol 90:8954 –8967.;doi:10.1128/JVI.00612-16.
Abstract: Interleukin 2 (IL-2) signaling through the IL-2 receptor alpha chain (CD25) facilitates HIV replication in vitro and facilitates homeostatic proliferation of CD25 FoxP3 CD4 T cells. CD25 FoxP3 CD4 T cells may therefore constitute a suitable subset for HIV infection and plasma virion production. CD25 FoxP3 CD4 T cell frequencies, absolute numbers, and the expression of CCR5 and cell cycle marker Ki67 were studied in peripheral blood from HIV and HIV study volunteers. Different memory CD4 T cell subsets were then sorted for quantification of cell-associated HIV DNA and phylogenetic analyses of the highly variable EnvV1V3 region in comparison to plasma-derived virus sequences. In HIV subjects, 51% (median) of CD25 FoxP3 CD4 T cells expressed the HIV coreceptor CCR5. Very high frequencies of Ki67 cells were detected in CD25 FoxP3 memory CD4 T cells (median, 27.6%) in comparison to CD25 FoxP3 memory CD4 T cells (median, 4.1%; P<0.0001). HIV DNA content was 15-fold higher in CD25 FoxP3 memory CD4 T cells than in CD25 FoxP3 T cells (P 0.003). EnvV1V3 sequences derived from CD25 FoxP3 memory CD4 T cells did not preferentially cluster with plasma-derived sequences. Quasi-identical cell-plasma sequence pairs were rare, and their proportion decreased with the estimated HIV infection duration. These data suggest that specific cellular characteristics of CD25 FoxP3 memory CD4 T cells might facilitate efficient HIV infection in vivo and passage of HIV DNA to cell progeny in the absence of active viral replication. The contribution of this cell population to plasma virion production remains unclear.
Description: An article published by Chachage M, Pollakis G, Kuffour EO, Haase K, Bauer A, Nadai Y, Podola L, Clowes P, Schiemann M, Henkel L, Hoffmann D, Joseph S, Bhuju S, Maboko L, Sarfo FS, Eberhardt K, Hoelscher M, Feldt T, Saathoff E, Geldmacher C. 2016. CD25 FoxP3 memory CD4 T cells are frequent targets of HIV infection in vivo. J Virol 90:8954 –8967; doi:10.1128/JVI.00612-16.
URI: http://hdl.handle.net/123456789/13412
Appears in Collections:College of Health Sciences

Files in This Item:

File Description SizeFormat
Journal of Virology-2016-Chachage-8954.full.pdf2.13 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback