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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/13409

Title: Sensitivity of PCR Targeting Mycobacterium ulcerans by Use of Fine-Needle Aspirates for Diagnosis of Buruli Ulcer
Authors: Phillips, Richard Odame
Sarfo, Fred Stephen
Osei-Sarpong, F.
Boateng, A.
Tetteh, I.
Lartey, A.
Adentwe, E.
Opare, W.
Asiedu, K. B.
Wansbrough-Jones, Mark
Issue Date: Apr-2009
Publisher: Journal of Clinical Microbiology
Citation: Journal of Clinical Microbiology,Apr. 2009, p. 924–926, Vol. 47, No. 4 0095-1137/09/$08.00 0 doi:10.1128/JCM.01842-08
Abstract: In a previous study, we reported that the sensitivity of PCR targeting the IS2404 insertion sequence of Mycobacterium ulcerans was 98% when it was applied to 4-mm punch biopsy samples of Buruli lesions. Fine-needle aspiration (FNA) is a less traumatic sampling technique for nonulcerated lesions, and we have studied the sensitivity of PCR using FNA samples. Fine-needle aspirates were taken with a 21-gauge needle from 43 patients diagnosed clinically with M. ulcerans disease. Four-millimeter punch biopsies were obtained for microscopy, culture, and PCR targeting the IS2404 insertion sequence. The sensitivity of PCR using samples obtained by FNA was 86% (95% confidence interval [95% CI], 72 to 94%) compared with that for PCR using punch biopsy samples. In this study, the sensitivities of culture and microscopy for punch biopsy samples were 44% (95% CI, 29 to 60%) and 26% (95% CI, 14 to 41%), respectively. This demonstrates that PCR on an FNA sample is a viable minimally invasive technique to diagnose M. ulcerans lesions.
Description: An article published in Journal of Clinical Microbiology,Apr. 2009, p. 924–926, Vol. 47, No. 4 0095-1137/09/$08.00 0 doi:10.1128/JCM.01842-08
URI: http://hdl.handle.net/123456789/13409
Appears in Collections:College of Health Sciences

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