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|Title: ||Sensitivity of PCR Targeting Mycobacterium ulcerans by Use of Fine-Needle Aspirates for Diagnosis of Buruli Ulcer|
|Authors: ||Phillips, Richard Odame|
Sarfo, Fred Stephen
Asiedu, K. B.
|Issue Date: ||Apr-2009|
|Publisher: ||Journal of Clinical Microbiology|
|Citation: ||Journal of Clinical Microbiology,Apr. 2009, p. 924–926, Vol. 47, No. 4 0095-1137/09/$08.00 0 doi:10.1128/JCM.01842-08|
|Abstract: ||In a previous study, we reported that the sensitivity of PCR targeting the IS2404 insertion sequence of
Mycobacterium ulcerans was 98% when it was applied to 4-mm punch biopsy samples of Buruli lesions.
Fine-needle aspiration (FNA) is a less traumatic sampling technique for nonulcerated lesions, and we have
studied the sensitivity of PCR using FNA samples. Fine-needle aspirates were taken with a 21-gauge needle
from 43 patients diagnosed clinically with M. ulcerans disease. Four-millimeter punch biopsies were obtained
for microscopy, culture, and PCR targeting the IS2404 insertion sequence. The sensitivity of PCR using
samples obtained by FNA was 86% (95% confidence interval [95% CI], 72 to 94%) compared with that for PCR
using punch biopsy samples. In this study, the sensitivities of culture and microscopy for punch biopsy samples
were 44% (95% CI, 29 to 60%) and 26% (95% CI, 14 to 41%), respectively. This demonstrates that PCR on an
FNA sample is a viable minimally invasive technique to diagnose M. ulcerans lesions.|
|Description: ||An article published in Journal of Clinical Microbiology,Apr. 2009, p. 924–926, Vol. 47, No. 4 0095-1137/09/$08.00 0 doi:10.1128/JCM.01842-08|
|Appears in Collections:||College of Health Sciences|
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