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|Title: ||Next-Generation Sequencing Reveals Frequent Opportunities for Exposure to Hepatitis C Virus in Ghana|
|Authors: ||Forbi, Joseph C.|
Layden, Jennifer E.
Phillips, Richard Odame
Campo, David S.
Sarfo, Fred Stephen...et.al
|Issue Date: ||2015|
|Publisher: ||Plos one|
|Citation: ||Forbi JC, Layden JE, Phillips RO, Mora N, Xia G-l, Campo DS, et al. (2015) Next-Generation Sequencing Reveals Frequent Opportunities for Exposure to Hepatitis C Virus in Ghana. PLoS ONE 10(12): e0145530. doi:10.1371/journal.pone.0145530|
|Abstract: ||Globally, hepatitis C Virus (HCV) infection is responsible for a large proportion of persons
with liver disease, including cancer. The infection is highly prevalent in sub-Saharan Africa.
West Africa was identified as a geographic origin of two HCV genotypes. However, little is
known about the genetic composition of HCV populations in many countries of the region.
Using conventional and next-generation sequencing (NGS), we identified and genetically
characterized 65 HCV strains circulating among HCV-positive blood donors in Kumasi,
Ghana. Phylogenetic analysis using consensus sequences derived from 3 genomic regions
of the HCV genome, 5'-untranslated region, hypervariable region 1 (HVR1) and NS5B
gene, consistently classified the HCV variants (n = 65) into genotypes 1 (HCV-1, 15%) and
genotype 2 (HCV-2, 85%). The Ghanaian and West African HCV-2 NS5B sequences were
found completely intermixed in the phylogenetic tree, indicating a substantial genetic heterogeneity
of HCV-2 in Ghana. Analysis of HVR1 sequences from intra-host HCV variants
obtained by NGS showed that three donors were infected with >1 HCV strain, including
infections with 2 genotypes. Two other donors share an HCV strain, indicating HCV transmission
between them. The HCV-2 strain sampled from one donor was replaced with
another HCV-2 strain after only 2 months of observation, indicating rapid strain switching.
Bayesian analysis estimated that the HCV-2 strains in Ghana were expanding since the
16th century. The blood donors in Kumasi, Ghana, are infected with a very heterogeneous
HCV population of HCV-1 and HCV-2, with HCV-2 being prevalent. The detection of three
cases of co- or super-infections and transmission linkage between 2 cases suggests frequent
opportunities for HCV exposure among the blood donors and is consistent with the
reported high HCV prevalence. The conditions for effective HCV-2 transmission existed
for ~ 3–4 centuries, indicating a long epidemic history of HCV-2 in Ghana.|
|Description: ||An article published by Forbi JC, Layden JE, Phillips RO, Mora N,
Xia G-l, Campo DS, et al. (2015) Next-Generation
Sequencing Reveals Frequent Opportunities for
Exposure to Hepatitis C Virus in Ghana. PLoS ONE
10(12): e0145530. doi:10.1371/journal.pone.0145530|
|Appears in Collections:||College of Health Sciences|
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