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|Title: ||Acalypha wilkesiana ‘inferno’ hydroethanolic leaf extract has protective effect on carbon tetrachloride-induced subacute toxicity in animals|
|Authors: ||Larbie, Christopher|
Emikpe, Benjamin O.
Oyagbemi, Ademola A.
Nyarko, Ruby A.
Jarikre, Theophilus A.
Adjei, Clement O.
Aseidu, Emmanuel B.
|Keywords: ||Acalypha wilkesiana inferno|
|Issue Date: ||5-May-2020|
|Publisher: ||Biomedical Research and Therapy|
|Citation: ||Biomedical Research and Therapy, 7(5):3778-3788|
|Abstract: ||Introduction: Liver fibrosis is one of the most common clinical manifestations of hepatic diseases.
However, though many synthetic drugs exist for the treatment and prevention of liver diseases,
liver injuries still persist. The present study, therefore, sought to investigate the subacute protective
effects of Acalphya wilkesiana against carbon tetrachloride (CCl4)-induced toxicity in animals.
Methodology: Liver injury was induced in experimental animals by administering CCl4 (1:1 v/v
in olive oil, intraperitoneally (i.p.), twice weekly for 8 weeks) after pre-treatment with extract of A.
wilkesiana (AWE). AWE (250 mg/kg) and Silymarin (120 mg/kg) were administered orally (daily for 8
weeks). The hepatoprotective effect was studied by assaying the activity of liver enzymes, such as
alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and
alpha-fetoprotein. The effect of the treatments on liver prooxidants (e.g. malondialdehyde [MDA])
and antioxidants (e.g. superoxide dismutase [SOD], reduced glutathione [GSH], glutathione peroxidase
[GPx], and glutathione transferase [GST]), as well as inflammatory cytokines (e.g. interleukin
[IL]-17, IL-23, nuclear factor kappa beta [NF-kB], and cycloxygenase-1 [COX-1]) and the histology
of the liver were also examined. Results: The activity of liver function biomarkers changed significantly
upon CCl4 administration; increases in ALT, total and direct bilirubin, and some fibrosis
indices (e.g. alpha-fetoprotein and APRI [p<0.05-0.001, compared with normal]) were observed.
Co-administration of AWE with CCl4 restored these to normal levels. The intensity of structural alterations
revealed that the AWE treatment has protective potential against subacute liver injury.
AWE treatment also reduced the expression of IL-17, 1L-23, NF-kB and COX-1, underscoring its antiinflammatory
properties. Conclusion: The results of the current study generally suggest that hydroethanolic
leaf extracts of A. wilkesiana inferno possess some subacute protective activity by improving
liver function and inhibition of inflammation, and could be developed as a potent antifibrotic
|Description: ||This article has been published in Biomedical Research and Therapy and is available at DOI : 10.15419/bmrat.v7i5.605|
|Appears in Collections:||College of Science|
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