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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/13023

Title: Antimicrobial resistance (AMR) and molecular characterization of Neisseria gonorrhoeae in Ghana, 2012-2015
Authors: Attram, Naiki
Agbodzi, Bright
Dela, Helena
Behene, Eric
Nyarko, Edward O
Kyei, Nicholas N. A
Larbi, John A
Lawson, Bernard W. L
Addo, Kennedy K.
Newman, Mercy J. et...al
Issue Date: Oct-2019
Publisher: PLOS ONE
Citation: PLOS ONE
Abstract: Neisseria gonorrhoeae antimicrobial resistance (AMR) surveillance is essential for tracking the emergence and spread of AMR strains in local, national and international populations. This is crucial for developing or refining treatment guidelines. N. gonorrhoeae multiantigen sequence typing (NG-MAST) is beneficial for describing the molecular epidemiology of gonococci at national and international levels. Elucidation of AMR determinants to β-lactam drugs, is a means of monitoring the development of resistance. In Ghana, little is known about the current gonococcal AMR prevalence and no characterization of gonococcal isolates has been previously performed. In this study, gonococcal isolates (n = 44) collected from five health facilities in Ghana from 2012 to 2015, were examined using AMR testing, NG-MAST and sequencing of penA. High rates of resistance were identified to tetracycline (100%), benzylpenicillin (90.9%), and ciprofloxacin (81.8%). One isolate had a high cefixime MIC (0.75 μg/ml). Twenty-eight NG-MAST sequence types (STs) were identified, seventeen of which were novel. The isolate with the high cefixime MIC contained a mosaic penA-34 allele and belonged to NG-MAST ST1407, an internationally spreading multidrug-resistant clone that has accounted for most cefixime resistance in many countries. In conclusion, AMR testing, NG-MAST, and sequencing of the AMR determinant penA, revealed high rates of resistance to tetracycline, benzylpenicillin, and ciprofloxacin; as well as a highly diverse population of N. gonorrhoeae in Ghana. It is imperative to continue with enhancedAMR surveillance and to understand the molecular epidemiology of gonococcal strains circulating in Ghana and other African countries
Description: This article is published in PLOS ONE and also available at https://doi.org/10.1371/journal.pone.0223598
URI: 10.1371/journal.pone.0223598
Appears in Collections:College of Science

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