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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12923

Title: Peptide Mix from Olivancillaria hiatula Interferes with Cell-toCell Communication in Pseudomonas aeruginosa
Authors: Gasu, Edward Ntim
Ahor, Hubert Senanu
Borquaye, Lawrence Sheringham
Issue Date: 24-Sep-2019
Publisher: Hindawi
Abstract: Bacteria in biofilms are encased in an extracellular polymeric matrix that limits exposure of microbial cells to lethal doses of antimicrobial agents, leading to resistance. In Pseudomonas aeruginosa, biofilm formation is regulated by cell-to-cell communication, called quorum sensing. Quorum sensing facilitates a variety of bacterial physiological functions such as swarming motility and protease, pyoverdine, and pyocyanin productions. Peptide mix from the marine mollusc, Olivancillaria hiatula, has been studied for its antibiofilm activity against Pseudomonas aeruginosa. Microscopy and microtiter plate-based assays were used to evaluate biofilm inhibitory activities. Effect of the peptide mix on quorum sensing-mediated processes was also evaluated. Peptide mix proved to be a good antibiofilm agent, requiring less than 39 μg/mL to inhibit 50% biofilm formation. Micrographs obtained confirmed biofilm inhibition at 1/2 MIC whereas 2.5 mg/mL was required to degrade preformed biofilm. ,ere was a marked attenuation in quorum sensing-mediated phenotypes as well. At 1/2 MIC of peptide, the expression of pyocyanin, pyoverdine, and protease was inhibited by 60%, 72%, and 54%, respectively. Additionally, swarming motility was repressed by peptide in a dose-dependent manner. ,ese results suggest that the peptide mix from Olivancillaria hiatula probably inhibits biofilm formation by interfering with cell-to-cell communication in Pseudomonas aeruginosa.
Description: An article published by Hindawi and also available at https://doi.org/10.1155/2019/5313918
URI: http://hdl.handle.net/123456789/12923
Appears in Collections:College of Health Sciences

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