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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12747

Title: Identification of New Genes Involved in mtDNA Maintenance in Caenorhabditis elegansthat could Represent Candidate Genes for Mitochondrial Diseases
Authors: Addo, Matthew Glover
Cossard, Richard
Pichard, Damien
Obiri-Danso, Kwasi
Rötig, Agnes
et. al
Keywords: candidate genes
mtDNA
EtBr
orthologs
mitochondrial
diseases
Issue Date: 10-Jun-2016
Publisher: Int.J.Curr.Microbiol.App.Sci.
Citation: Matthew Glover Addo, Richard Cossard, Damien Pichard, Kwasi Obiri-Danso, Agnes Rötig and Agnes Delahodde. 2016. Identification of New Genes Involved in mtDNA Maintenance in Caenorhabditis elegansthat could Represent Candidate Genes for Mitochondrial Diseases. Int.J.Curr.Microbiol.App.Sci.5(6): 179-189. doi: http://dx.doi.org/10.20546/ijcmas.2016.506.022
Abstract: Identification of genes involved inmtDNA maintenance in Caenorhabditis elegans that could represent candidate genes for mitochondrial diseases associated with quantitative orqualitative mtDNA abnormalities was performed using our previous validated method of RNAi combined with ethidium bromide. This was to knock down C. elegansgenes homologous to human genes known to be involved in mtDNA stability. Silencing of these candidate genes were repeated again without EtBr to measure the worm’s mtDNA content and the efficiency of gene silencing by Q-PCR and RT-Q-PCR. Among the 182 genes encoding mitochondrial proteins tested randomly, we found that inactivation of 25 of them lead to EtBr sensitivity. Out of these genes, 20 fall into four main categories of metabolism (GOT2, WWOX, ALDH1L2, WWOX, AGMAT and ACOT9), protein synthesis (TRMU, MRPL47, MRPS14,MRPS18C, MRPL4and MRPL12), mitochondrial morphology (OPA1,)and respiratory chain oxidative phosphorylation (ATP5F1, NDUFS4, FH, CYP2C8, CYP3A7, CYP2C8 and CYP2C8).The human orthologs of these genes as presented in this study could be considered as reservoir of new genes which will represent a helpful tool and knowledge for mitochondrial diseases, as they would be considered as candidate genes for patients.
Description: An article published by International Journal of Current Microbiology and Applied Sciences and also available at http://dx.doi.org/10.20546/ijcmas.2016.506.022
URI: http://hdl.handle.net/123456789/12747
Appears in Collections:College of Science

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