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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12739

Title: Investigation of yeast genes possibly involved in mtDNA stability using the nematode Caenorhabditis elegans
Authors: Addo, Matthew Glover
Cossard, R.
Pichard, D.
Obiri-Danso, K.
Rötig, A.
et. al
Keywords: mtDNA
Caenorhabditis elegans
nucleoid
RNAi
candidate genes
homolog
MIRO1
Issue Date: 6-May-2016
Publisher: Academic Journals
Abstract: Screening of Caenorhabditis elegansgenes possibly involved in the mitochondrial genome maintenance was performed using our previous validated method of RNAi combined with ethidium bromide. This was to knock down C. elegansgenes homologous to yeast genes known to be involved in mtDNA stability but of unknown molecular function or to identify transient components that could play important role on the stability of mtDNA in a temporal and/or spatial manner. C. eleganshomologs for 11 genes among 27 yeast genes for which deletion leads to a rho0 state were found, however, only 5 genes were present in the RNAi library. Out of these 5 genes, 1 gene (homolog of GEM1) gave a clear L3 arrest on RNAi and ethidium bromide indicating its involvement on mtDNA stability. Four other genes homologs of MTG2, YER087W, AVL9andRRG3did not lead to L3 arrest even though their deletion in Saccharomyces cerevisiaeleads to rho0 state. Although MTG2has been reported to be important in the function and structure on mtDNA stability in yeast, our results did not support those findings in C. elegans. The human homolog of this gene (MIRO1) can be considered as a candidate gene involved in mtDNA stability and sequenced in patients with mtDNA depletion diseases
Description: An article published by Academic Journals and also available at DOI: 10.5897/AJB2016.15208
URI: http://hdl.handle.net/123456789/12739
Appears in Collections:College of Science

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