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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12719

Title: The therapeutic landscape of HIV-1 via genome editing
Authors: Kwarteng, Alexander
Ahuno, Samuel Terkper
Kwakye‑Nuako, Godwin
Issue Date: 14-Jul-2017
Publisher: Springer Nature
Abstract: Current treatment for HIV‑1 largely relies on chemotherapy through the administration of antiretroviral drugs. While the search for anti‑HIV‑1 vaccine remain elusive, the use of highly active antiretroviral therapies (HAART ) have been far‑reaching and has changed HIV‑1 into a manageable chronic infection. There is compelling evidence, including sev‑eral side‑effects of ARTs, suggesting that eradication of HIV‑1 cannot depend solely on antiretrovirals. Gene therapy, an expanding treatment strategy, using RNA interference (RNAi) and programmable nucleases such as meganuclease, zinc finger nuclease (ZFN), transcription activator‑like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats/CRISPR‑associated proteins (CRISPR–Cas9) are transforming the therapeutic landscape of HIV‑1. TALENS and ZFNS are structurally similar modular systems, which consist of a FokI endonuclease fused to custom‑designed effector proteins but have been largely limited, particularly ZFNs, due to their complexity and cost of protein engineering. However, the newly developed CRISPR–Cas9 system, consists of a single guide RNA (sgRNA), which directs a Cas9 endonuclease to complementary target sites, and serves as a superior alternative to the previous protein‑based systems. The techniques have been successfully applied to the development of better HIV‑1 models, generation of protective mutations in endogenous/host cells, disruption of HIV‑1 genomes and even reactivating latent viruses for better detection and clearance by host immune response. Here, we focus on gene editing‑based HIV‑1 treatment and research in addition to providing perspectives for refining these techniques.
Description: An article published by Springer Nature and also available at DOI 10.1186/s12981-017-0157-8
URI: http://hdl.handle.net/123456789/12719
Appears in Collections:College of Science

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