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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12717

Title: Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells
Authors: Katawa, Gnatoulma
Layland, Laura E.
Debrah, Alex Y.
Horn, Charlotte von
Kwarteng, Alexander
et. al
Issue Date: 8-Jan-2015
Publisher: PLoS Negl Trop Dis
Citation: Katawa G, Layland LE, Debrah AY, von Horn C, Batsa L, et al. (2015) Hyperreactive Onchocerciasis is Characterized by a Combination of Th17-Th2Immune Responses and Reduced Regulatory T Cells. PLoS Negl Trop Dis 9(1): e3414. doi:10.1371/journal.pntd.0003414
Abstract: Clinical manifestations in onchocerciasis range from generalized onchocerciasis (GEO) to the rare but severe hyperreactive(HO)/sowda form. Since disease pathogenesis is associated with host inflammatory reactions, we investigated whether Th17responses could be related to aggravated pathology in HO. Using flow cytometry, filarial-specific cytokine responses andPCR arrays, we compared the immune cell profiles, including Th subsets, in individuals presenting the two polar forms ofinfection and endemic normals (EN). In addition to elevated frequencies of memory CD4+T cells, individuals with HOshowed accentuated Th17 and Th2 profiles but decreased CD4+CD25hiFoxp3+regulatory T cells. These profiles includedincreased IL-17A+, IL-4+, RORC2+and GATA3+CD4+T cell populations. Flow cytometry data was further confirmed using aPCR array since Th17-related genes (IL-17 family members, IL-6, IL-1band IL-22) and Th2-related (IL-4, IL-13, STAT6) geneswere all significantly up-regulated in HO individuals. In addition, strongerOnchocerca volvulus-specific Th2 responses,especially IL-13, were observedin vitroin hyperreactive individuals when compared to GEO or EN groups. This studyprovides initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating thedevelopment of severe onchocerciasis.
Description: An article published by PLoS Negl Trop Dis and also available at doi:10.1371/journal.pntd.0003414
URI: http://hdl.handle.net/123456789/12717
Appears in Collections:College of Science

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