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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12708

Title: Transcriptomics and Immune Profilesof Asymptomatic Filarial-Infected Individuals
Authors: Kwarteng, Alexander
Issue Date: Aug-2015
Abstract: Filarial infections caused by Wuchereria bancroftiand Brugiaspecies (lymphatic filariasis (LF)) and Onchocerca volvulus(onchocerciasis) affect almost 200 million individuals worldwide and pose major public health challengesinendemic regions. Indeed, the collective DALYs (disability-adjusted life years) for both infections is 3.3 million. Infections with these thread-like nematodes are chronic and although most individuals develop a regulated state, a portion develop severe forms of pathology. Mass drug administration (MDA) programmes on endemic populations focus on reducing prevalencelevels of people with microfilariae (MF), the worm's offspringin the bloodto less than 1%. Although this has been successful in some areas, studies show that MDA will be required for longer than initially conceived. Thus, there is still a requirement for better drugs or vaccines.W. bancrofti-infected individuals without pathology (asymptomatic) can be subdivided into two groupsthat are patent (MF+) or latent (MF-). Patent infections are associated with an immunologically tolerant phenotype state that favours worm survival and in addition does not provoke overt pathology in the host. Latent infections are characterized by the lack of MF in the periphery, despite the presence of adult worms, and their immune profiles show markers of immune-mediated MF control. In O. volvulusinfection however, the majority of individuals havedermal-residing MFand amicrofilaridermic (a-MF) individuals appear to be the consequences of repeated MDA treatment. Interestingly, recent research revealed that O. volvulusendemic areas,with a lowered infection pressure due to MDA,appear to influencebystander responsesto Plasmodium-derived antigens in community members even if they have not regularlyparticipated in the therapy.Pathology that arises in either filarial infection is associated with dampened regulatory T cell responses (Treg) and IL-10 but elevated Th17 responses. Thus, identifying immune determinants that drive these different infection stateshas the potential to guide the development of improvedanti-filarial drugsand vaccines. In this study, microarray and cellular profiling approaches were used to evaluate gene expression patterns and to revealgenetic pathways specific to W. bancroftior O. volvulusinfection. Individuals with latentLFinfections showed an enhanced gene expressionprofile, including genes involved in Actin Nucleation by ARP-WASP Complex, Rac signaling, Cdc42 signaling, RhoGD1 signaling, eosinophil effector functions and CD28 signaling in T helper cellpathways. Interestingly, the Charcot-Leyden crystal/galectin-10(CLC/Gal-10), an immunosuppressive molecule,was among the top commonly expressed genes in both infections and elevated levels were also detected in plasma.Moreover, compared to healthy volunteers, T cells recovered from W. bancrofti-infected individuals secreted higher levels of CLC/Gal-10and were even higher in MF+ individuals: by complementingtheir elevated Treg responses (Foxp3/IL-10). LatentW. bancrofti-infected individualson the other hand had pronounced Th1, Th2 and Th17 responses. With regards to filarial-specificantibody responses, IgG4, IgE and IgA in plasma were associated with MF+, MF-and endemic normals, respectively. Overall, the transcriptome profiling revealed overlappinggenes in both infections: CLC/Gal-10, ribonuclease RNase A family, 2(RNASE2) and ribosomal protein S4, y-linked 1(RPS4Y1). Thus, the study offersinsightinto filarial-specific genes, signaling pathways and ivimmune determinants, which may be central targets towards the development of new anti-filarial interventions.
URI: http://hdl.handle.net/123456789/12708
Appears in Collections:College of Science

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