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|Title: ||Optimization of protein extraction from fern frond using response surface methodology (RSM)|
|Authors: ||Essuman, E. K.|
Ankar-Brewoo, Gloria Mathanda
Ofosu, I. W.
Response Surface Methodology
|Issue Date: ||2014|
|Publisher: ||Ife Journal of Science|
|Citation: ||Essuman et al.: Optimization of Protein Extraction from Fern Frond|
|Abstract: ||Protein extractability from Nephrolepis biserrata and Arthropteris orientalis was studied under various conditions.
The extraction of fern protein was conducted using alkaline, alcohol and saline treatments under various
conditions of treatment concentrations and time of agitation. Central composite design of Response Surface
Methodology (RSM) was used for identification of the best condition and extraction yield optimization. Results
showed that alkaline treatment produced the highest extraction yield with maximum protein recovery of 5.11
mg/mL and 2.03 mg/mL for Nephrolepis biserrata and Arthropteris orientalis respectively at 0.1 M NaOH
concentration and 30 min of agitation time.|
|Description: ||An article published by Ife Journal of Science|
|Appears in Collections:||College of Science|
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