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|Title: ||Human coronaviruses associated with upper respiratory tract infections in three rural areas of Ghana|
|Authors: ||Owusu, Michael|
Sylverken, Augustina Angelina
Corman, Victor Max
|Issue Date: ||31-Jul-2014|
|Publisher: ||PLOS ONE|
|Citation: ||Owusu M, Annan A, Corman VM, Larbi R, Anti P, et al. (2014) Human Coronaviruses Associated with Upper Respiratory Tract Infections in Three Rural Areas of Ghana. PLoS ONE 9(7): e99782. doi:10.1371/journal.pone.0099782|
|Abstract: ||Background: Acute respiratory tract infections (ARI) are the leading cause of morbidity and mortality in developing
countries, especially in Africa. This study sought to determine whether human coronaviruses (HCoVs) are associated with
upper respiratory tract infections among older children and adults in Ghana.
Methods: We conducted a case control study among older children and adults in three rural areas of Ghana using
asymptomatic subjects as controls. Nasal/Nasopharyngeal swabs were tested for Middle East respiratory syndrome
coronavirus (MERS-CoV), HCoV-22E, HCoV-OC43, HCoV-NL63 and HCoV-HKU1 using Reverse Transcriptase Real-Time
Polymerase Chain Reaction.
Results: Out of 1,213 subjects recruited, 150 (12.4%) were positive for one or more viruses. Of these, single virus detections
occurred in 146 subjects (12.0%) and multiple detections occurred in 4 (0.3%). Compared with control subjects, infections
with HCoV-229E (OR = 5.15, 95%CI = 2.24–11.78), HCoV-OC43 (OR = 6.16, 95%CI = 1.77–21.65) and combine HCoVs
(OR = 2.36, 95%CI = 1.5 = 3.72) were associated with upper respiratory tract infections. HCoVs were found to be seasonally
dependent with significant detections in the harmattan season (mainly HCoV-229E) and wet season (mainly HCoV-NL63). A
comparison of the obtained sequences resulted in no differences to sequences already published in GenBank.
Conclusion: HCoVs could play significant role in causing upper respiratory tract infections among adults and older children
in rural areas of Ghana.|
|Description: ||An article published by PLOS ONE and also available at doi:10.1371/journal.pone.0099782|
|Appears in Collections:||College of Science|
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