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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12361

Title: Plant-Based Production of Recombinant Plasmodium Surface Protein Pf38 and Evaluation of its Potential as a Vaccine Candidate
Authors: Feller, Tatjana
Thom, Pascal
Koch, Natalie
Spiegel, Holger
Addai-Mensah, Otchere
et. al
Issue Date: 21-Nov-2013
Publisher: Public Library of Science
Citation: Feller T, Thom P, Koch N, Spiegel H, Addai-Mensah O, et al. (2013) Plant-Based Production of Recombinant Plasmodium Surface Protein Pf38 and Evaluation of its Potential as a Vaccine Candidate. PLoS ONE 8(11): e79920. doi:10.1371/journal.pone.0079920
Abstract: Pf38 is a surface protein of the malarial parasite Plasmodium falciparum. In this study, we produced and purified recombinant Pf38 and a fusion protein composed of red fluorescent protein and Pf38 (RFP-Pf38) using a transient expression system in the plant Nicotiana benthamiana. To our knowledge, this is the first description of the production of recombinant Pf38. To verify the quality of the recombinant Pf38, plasma from semi-immune African donors was used to confirm specific binding to Pf38. ELISA measurements revealed that immune responses to Pf38 in this African subset were comparable to reactivities to AMA-1 and MSP119. Pf38 and RFP-Pf38 were successfully used to immunise mice, although titres from these mice were low (on average 1:11.000 and 1:39.000, respectively). In immune fluorescence assays, the purified IgG fraction from the sera of immunised mice recognised Pf38 on the surface of schizonts, gametocytes, macrogametes and zygotes, but not sporozoites. Growth inhibition assays using aPf38 antibodies demonstrated strong inhibition ($60%) of the growth of blood-stage P. falciparum. The development of zygotes was also effectively inhibited by aPf38 antibodies, as determined by the zygote development assay. Collectively, these results suggest that Pf38 is an interesting candidate for the development of a malaria vaccine.
Description: An article published by Public Library of Science and also available at doi:10.1371/journal.pone.0079920
URI: http://hdl.handle.net/123456789/12361
Appears in Collections:College of Health Sciences

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