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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12079

Title: ANTI-INFLAMMATORY, ANTIOXIDANT, AND SELECTIVE ANTIBACTERIAL EFFECTS OF EUADENIA EMINENS ROOT BARK.
Authors: Dickson, Rita A.
Fleischer, TC
Ekuadzi, E
Komlaga, G.
Keywords: Anti-infective
Anti-inflammatory
Antioxidan
Carrageenan-induced oedema
Euadenia eminens
Issue Date: Jan-2012
Publisher: Afr J Tradit Complement Altern Med.
Citation: Afr J Tradit Complement Altern Med., (2012) 9(2):271‐276 http://dx.doi.org/10.4314/ajtcam.v9i2.14 271
Abstract: Euadenia eminens Hook f. (Capparaceae) has traditional uses in the management of conjunctivitis, iritis, ophthalmia, tuberculosis, otalgia and rectal prolapse. The fruit pulp is also eaten as an aphrodisiac. In this paper, we report on the antiinflammatory, antioxidant and antibacterial effects of its roots. A 70 % ethanol extract was tested for anti-inflammatory effect using the carrageenan-induced oedema in chicks. Free radical scavenging, total antioxidant and total phenol content were assessed spectrophotometrically. The extract was tested for antibacterial activity using the agar well diffusion method and micro dilution assays. The 70% ethanol extract gave a maximal inhibition of oedema by 74.18 % at 30 mg/kg. The total antioxidant capacity expressed in terms of ascorbic acid was 0.609 mg/g dry weight. The total phenol in terms of tannic acid was 7.25 mg/g dry weight. The extract also demonstrated free radical scavenging activity yielding IC50 value of 1.175 mg/mL. The root extract however, showed selective antibacterial activity, inhibiting growth of two microorganisms; Bacillus subtilis and Bacillus thurigiensis. The MICs were 500 and 1000 µg/mL respectively. These results may account in part for the ethnopharmacological use of the plant.
Description: This article is published in Afr J Tradit Complement Altern Med.and also available at http://dx.doi.org/10.4314/ajtcam.v9i2.14
URI: http://dx.doi.org/10.4314/ajtcam.v9i2.14 271 ANTI-INFLAMMATORY, ANTIOXIDANT, AND SELECTIVE ANTIBACTERIAL EFFECTS OF EUADENIA EMINENS ROOT BARK. Dickson RA1*, Fleischer TC2 , Ekuadzi E1, G. Komlaga1 1 Department of Pharmacognosy, 2 Department of Herbal Medicine, Faculty of Pharmacy and Pharmaceutical Sciences, KNUST, Kumasi. *R.A. Dickson, Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi-Ghana. E-mail: radickson.pharm@knust.edu.gh Abstract Euadenia eminens Hook f. (Capparaceae) has traditional uses in the management of conjunctivitis, iritis, ophthalmia, tuberculosis, otalgia and rectal prolapse. The fruit pulp is also eaten as an aphrodisiac. In this paper, we report on the antiinflammatory, antioxidant and antibacterial effects of its roots. A 70 % ethanol extract was tested for anti-inflammatory effect using the carrageenan-induced oedema in chicks. Free radical scavenging, total antioxidant and total phenol content were assessed spectrophotometrically. The extract was tested for antibacterial activity using the agar well diffusion method and micro dilution assays. The 70% ethanol extract gave a maximal inhibition of oedema by 74.18 % at 30 mg/kg. The total antioxidant capacity expressed in terms of ascorbic acid was 0.609 mg/g dry weight. The total phenol in terms of tannic acid was 7.25 mg/g dry weight. The extract also demonstrated free radical scavenging activity yielding IC50 value of 1.175 mg/mL. The root extract however, showed selective antibacterial activity, inhibiting growth of two microorganisms; Bacillus subtilis and Bacillus thurigiensis. The MICs were 500 and 1000 µg/mL respectively. These results may account in part for the ethnopharmacological use of the plant. Key words: Anti-infective, Anti-inflammatory, Antioxidant, Carrageenan-induced oedema, Euadenia eminens. Introduction Medicinal plants represent a largely untapped source of molecules with diverse structures. These bioactive molecules are a unique source of potentially new drug leads necessary to combat the increasing health challenges confronting the world. Antibiotic resistance is one such major threat to public health (Smith et al., 1991). Multi-drug resistance, as well as the emergence of new infectious diseases have prompted the discovery and development of new drug molecules which are not simply based on existing antibiotic templates. Furthermore, the management of chronic inflammatory disorders poses an uncertain outcome. Chronic inflammatory disorders: chronic non-healing ulcers, rheumatoid arthritis, chronic obstructive pulmonary disease, etc., remain one of the major health challenges facing the world (Li et al., 2003). Inflammation is a component of almost every disease condition including arthritis, bronchitis, iritis, etc., and if unchecked would turn chronic. The relevance of African traditional medicine in the management of various ailments has been well established (Soforowa, 1982). Globally, attention has been turned to herbal medicines; a trend recognized and advocated by the World Health Organization in its Alma Atta declaration for health for all. Medicinal plants are currently being scientifically investigated for their potential in the management of infectious and inflammatory disorders. Euadenia eminens Hook f. (Capparidaceae) was selected for this study based on its ethnopharmacological uses in Ghana. A shrub or small tree up to 5 m tall. The juice from cut fruit is used as drops for sore eyes, conjunctivitis, iritis, ophthalmia and trachoma. The fruit pulp is eaten as an aphrodisiac (Abbiw, 1990). Communications with some herbalists in Aburi, Ghana showed that the root decoction is used in tuberculosis, otalgia and rectal prolapse (Agbovie et al., 2002). However, no scientific investigations to the best of our knowledge of these effects have been undertaken. Therefore, the present work aimed to verify the anti-inflammatory, antibacterial and antioxidant effects of the root bark of this plant. Methods Plant collection T
http://hdl.handle.net/123456789/12079
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