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Title: Antiinflammatory and Analgesic Effects in Rodent Models of Ethanol Extract of Clausena anisata Roots and their Chemical Constituents
Authors: Dickson, Rita A.
Kumatia, Emmanuel Kofi
Annan, Kofi
Mensah, Abraham Yeboah
Amponsah, Isaac Kingsley
Appiah, Alfred A.
Hu Tung, Nguyen
Edoh, Dominic Adotey
Habtemariam, Solomon
Keywords: Clausena anisata roots
Opioid antagonist
Issue Date: Sep-2016
Publisher: Natural Product Communications
Citation: Natural Product Communications, 2017 Vol. 12 No. 1 67 - 72
Abstract: The in vivo anti-inflammatory and analgesic activities of the crude ethanol extract and chemical constituents of Clausena anisata roots were investigated. The crude extract, which was devoid of any visible acute toxicity, displayed significant anti-inflammatory effect at the dose of 1000 mg/kg (p.o.) when assessed using the carrageenan-induced oedema model. In the acetic acid-induced writhing and hot plate tests, it produced a very significant (p < 0.001), dose-dependent analgesic effect, with maximum analgesic activity of 72.1% at 1000 mg/kg (p.o.). Phytochemical analysis of the crude extract resulted in the isolation of four coumarins (anisocoumarin B, osthol, imperatorin and xanthotoxol) and a carbazole alkaloid, heptaphylline. Among the isolated compounds, osthol and anisocoumarin B produced the highest anti-inflammatory activity at 9 mg/kg (p.o.): slightly better than the positive control, indomethacin. Except for xanthotoxol, all the isolated compounds administered at 6 mg/kg (p.o.) produced significant analgesic activity and higher than diclofenac; with heptaphylline being the most potent (48.7%). The analgesic activity of anisocoumarin B (50.4%) was the highest among the isolates tested and the standard, tramadol, in the hot plate test. The nonselective opioid receptor antagonist, naloxone, abolished the analgesic effect of the crude extract and the tested isolates (anisocoumarin B and xanthotoxol) in the hot plate test suggesting an effect via the central opioidergic system. These findings provide the scientific basis for the use of C. anisata roots in traditional medicine as anti-inflammatory and analgesic agents.
Description: This article is published in Natural Product Communications and also available at https://doi.org/10.1177/1934578X1701200119
URI: 10.1177/1934578X1701200119
Appears in Collections:College of Health Sciences

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