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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/12018

Title: Linear epitopes in Onchocerca volvulus vaccine candidate proteins and excretory-secretory proteins
Authors: A.Y, Debrah
Lagatie, Ole
Verheyen, Ann
  Dorst, Bieke Van
  Debrah, Linda Batsa
  Stuyver, Lieven J.
Keywords: excretome/secretome proteins
linear epitope
Onchocerca volvulus
onchocerciasis
river blindness
serology
vaccine
Issue Date: Sep-2018
Publisher: Parasite Immunology
Abstract: In our previous study, a proteome‐wide screen was conducted to identify linear epitopes in this parasite's proteome, resulting in the discovery of three immunodominant motifs. Here we investigated whether such antigenic peptides were found in proteins that were already known as vaccine candidates and excretome/secretome proteins for O. volvulus. This approach led to the identification of 71 immunoreactive stretches in 46 proteins. A deep‐dive into the immunoreactivity profiles of eight vaccine candidates that were chosen as most promising candidates for further development (Ov‐CPI‐2, Ov‐ALT‐1, Ov‐RAL‐2, Ov‐ASP‐1, Ov‐103, Ov‐RBP‐1, Ov‐CHI‐1, and Ov‐B20), resulted in the identification of a poly‐glutamine stretch in Ov‐RAL‐2 that has properties for use as a serodiagnostic marker for O. volvulus infection. A peptide ELISA was developed, and the performance of this assay was evaluated. Based on this assessment, it was found that this assay has a sensitivity of 75.0% [95% CI: 64.9%‐83.5%] and a specificity of 98.5% [95% CI: 94.6%‐99.8%]. Furthermore, 8.7% reactivity in Asian parasite‐infected individuals (8 out of 92) was observed. Besides this identification of a linear epitope marker, the information on the presence of linear epitopes in vaccine candidate proteins might be useful in the study of vaccines for river blindness. This article is protected by copyright. All rights reserved.
Description: This article is published in Parasite Immunology and also available DOI: 10.1111/pim.12587
URI: 10.1111/pim.12587
http://hdl.handle.net/123456789/12018
Appears in Collections:College of Health Sciences

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