Research Articles >
College of Health Sciences >
Please use this identifier to cite or link to this item:
|Title: ||Performance evaluation of 3 serodiagnostic peptide epitopes and the derived multi-epitope peptide OvNMP-48 for detection of Onchocerca volvulus infection|
|Authors: ||Lagatie, Ole|
Debrah, Linda Batsa
Debrah, Yaw A.
Stuyver, Lieven J.
|Keywords: ||Onchocerca volvulus|
|Issue Date: ||May-2019|
|Publisher: ||Parasitology Research|
|Citation: ||Parasitology Research|
|Abstract: ||Current diagnostic tools to determine infection with the helminth parasite Onchocerca volvulus have limited performance
characteristics. In previous studies, a proteome-wide screen was conducted to identify linear epitopes in this parasite’s proteome,
resulting in the discovery of 1110 antigenic peptide fragments. Here, we investigated three of these peptides using peptide
ELISA’s and evaluated their sensitivity and specificity. Epitope mapping was performed, and peptides were constructed that
contained only the minimal epitope, flanked by a linker. Investigation of the performance of these minimal epitope peptides
demonstrated that all three of them have a specificity (as defined by lack of response in non-helminth-infected individuals) of
100%, low cross-reactivity (5.6%, 5.6%, and 9.3%, respectively), but low sensitivity (36.9%, 46.5%, and 41.2%, respectively).
Some cross-reactivity was observed in samples from individuals infected with soil-transmitted helminths or Brugia malayi.
Combining these three minimal epitopes in a single peptide, called OvNMP-48, resulted in a performance that exceeded the sum
of the individual epitopes, with a sensitivity of 76.0%, a specificity of 97.4%, and a cross-reactivity of 11.1%. Cross-reactivity
was observed in some STH and Brugia malayi-infected individuals. This work opens the opportunity to start exploring how these
novel linear epitope markers might become part of the O. volvulus diagnostic toolbox|
|Description: ||This article is published in Parasitology Research and also available at DOI: 10.1007/s00436-019-06345-3|
|Appears in Collections:||College of Health Sciences|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.