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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11975

Title: Evaluation of the diagnostic potential of urinary N-Acetyltyramine-O,β-glucuronide (NATOG) as diagnostic biomarker for Onchocerca volvulus infection
Authors: Lagatie, Ole
Ediage, Emmanuel Njumbe
Debrah, Linda Batsa
Diels, Luc
Nolten, Christ
Vinken, Petra
Debrah, Alex
Dillen, Lieve
Silber, Steven
Stuyver, Lieven J.
Keywords: Onchocerca volvulus
River blindness
Onchocerciasis
Biomarker
NATOG
Urine
Diagnostic
Issue Date: May-2016
Publisher: Parasites & Vectors
Citation: Parasites & Vectors 9(1)
Abstract: Background Onchocerciasis, also known as river blindness is one of the neglected tropical diseases affecting millions of people, mainly in sub-Saharan Africa and is caused by the filarial nematode Onchocerca volvulus. Efforts to eliminate this disease are ongoing and are based on mass drug administration programs with the microfilaricide ivermectin. In order to monitor the efficacy of these programs, there is an unmet need for diagnostic tools capable of identifying infected patients. We have investigated the diagnostic potential of urinary N-acetyltyramine-O,β-glucuronide (NATOG), which is a promising O. volvulus specific biomarker previously identified by urine metabolome analysis. MethodsA liquid chromatography tandem mass spectrometry (LC-MS/MS) method was used to assess the stability characteristics of NATOG and to evaluate the levels of NATOG in study samples. An LC-fluorescence method was also developed. ResultsStability characteristics of NATOG were investigated and shown to be ideally suited for use in tropical settings. Also, an easy and more accessible method based on liquid chromatography coupled to fluorescence detection was developed and shown to have the necessary sensitivity (limit of quantification 1 μM). Furthermore, we have evaluated the levels of NATOG in a population of 98 nodule-positive individuals from Ghana with no or low levels of microfilaria in the skin and compared them with the levels observed in different control groups (endemic controls (n = 50), non-endemic controls (n = 18) and lymphatic filariasis (n = 51). Only a few (5 %) of nodule-positive individuals showed an increased level (> 10 μM) of NATOG and there was no statistical difference between the nodule-positive individuals and the control groups (P > 0.05). Conclusions Results of the present study indicate the limited potential of NATOG as a diagnostic biomarker for O. volvulus infection in amicrofilaridermic individuals.
Description: This article is published in Parasites & Vectors and also available at DOI: 10.1186/s13071-016-1582-6
URI: 10.1186/s13071-016-1582-6
http://hdl.handle.net/123456789/11975
Appears in Collections:College of Health Sciences

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