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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11919

Title: Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay
Authors: Phillips, Richard O.
Frimpong, Michael
Ahor, Hubert Senanu
Wahed, Ahmed Abd El
Agbavor, Bernadette
et. al
Issue Date: 1-Feb-2019
Publisher: PLOS Neglected Tropical Diseases
Citation: PLoS Negl Trop Dis 13(2): e0007155. https://doi.org/10.1371/journal.pntd.0007155
Abstract: Access to an accurate diagnostic test for Buruli ulcer (BU) is a research priority according to the World Health Organization. Nucleic acid amplification of insertion sequence IS2404 by polymerase chain reaction (PCR) is the most sensitive and specific method to detect Mycobacterium ulcerans (M. ulcerans), the causative agent of BU. However, PCR is not always available in endemic communities in Africa due to its cost and technological sophistication. Isothermal DNA amplification systems such as the recombinase polymerase amplification (RPA) have emerged as a molecular diagnostic tool with similar accuracy to PCR but having the advantage of amplifying a template DNA at a constant lower temperature in a shorter time. The aim of this study was to develop RPA for the detection of M. ulcerans and evaluate its use in Buruli ulcer disease.
Description: An article published by PLOS Neglected Tropical Diseases and available at doi.org/10.1371/journal.pntd.0007155
URI: http://hdl.handle.net/123456789/11919
Appears in Collections:College of Health Sciences

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