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|Title: ||Loop-Mediated Isothermal Amplification for Laboratory Confirmation of Buruli Ulcer Disease—Towards a Point-of-Care Test|
|Authors: ||Beissner, Marcus|
Phillips, Richard O.
|Issue Date: ||13-Nov-2015|
|Publisher: ||PLOS Neglected Tropical Diseases|
|Citation: ||PLoS Negl Trop Dis 9(11): e0004219. doi:10.1371/journal.pntd.0004219|
|Abstract: ||As the major burden of Buruli ulcer disease (BUD) occurs in remote rural areas, development
of point-of-care (POC) tests is considered a research priority to bring diagnostic services
closer to the patients. Loop-mediated isothermal amplification (LAMP), a simple,
robust and cost-effective technology, has been selected as a promising POC test candidate.
Three BUD-specific LAMP assays are available to date, but various technical challenges
still hamper decentralized application. To overcome the requirement of cold-chains
for transport and storage of reagents, the aim of this study was to establish a dry-reagentbased
LAMP assay (DRB-LAMP) employing lyophilized reagents.|
|Description: ||An article published by PLOS Neglected Tropical Diseases|
|Appears in Collections:||College of Health Sciences|
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