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|Title: ||Development of a rapid, reliable and quantitative method — “SPOTi” for testing antifungal efficacy|
|Authors: ||Rizi, Khalida|
Danquah, Cynthia A.
|Issue Date: ||13-Jul-2015|
|Publisher: ||Journal of Microbiological Methods|
|Citation: ||Journal of Microbiological Methods 117 (2015) 36–40|
|Abstract: ||A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture
growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the antitubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and
broth micro-dilution anti-fungal assays to screen novel anti-fungals|
|Description: ||An article published by Journal of Microbiological Methods 117 (2015) 36–40|
|Appears in Collections:||College of Health Sciences|
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