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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11812

Title: Development of a rapid, reliable and quantitative method — “SPOTi” for testing antifungal efficacy
Authors: Rizi, Khalida
Murdan, Sudaxshina
Danquah, Cynthia A.
Faull, Jane
Bhakta, Sanjib
Keywords: SPOTi
Antifungals
Dermatophytes
Screening
Drug discovery
Issue Date: 13-Jul-2015
Publisher: Journal of Microbiological Methods
Citation: Journal of Microbiological Methods 117 (2015) 36–40
Abstract: A reference method for the antimicrobial susceptibility testing of common fungal pathogens such as dermatophytes, is currently lacking. In this study, we report the successful adaptation of solid agar-based spot culture growth inhibition assay (SPOTi) for dermatophytes, currently being used as a gold-standard in the antitubercular drug discovery field. The fungal-SPOTi assay correlated with the disc-diffusion method, and is validated using mycelial plugs. We propose the fungal-SPOTi as a high-throughput alternative to the disc-diffusion and broth micro-dilution anti-fungal assays to screen novel anti-fungals
Description: An article published by Journal of Microbiological Methods 117 (2015) 36–40
URI: http://hdl.handle.net/123456789/11812
Appears in Collections:College of Health Sciences

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