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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11155

Title: Gene cloning and characterization of a second alanine racemase from Bacillus subtilis encoded byyncD
Authors: Pierce, Kara J.
Salifu, Samson Pandam
Tangney, Martin
Keywords: Alanine racemase
Bacillus subtilis
Issue Date: 2008
Publisher: FEMS Microbiol Lett
Citation: FEMS Microbiol Lett, 283 (2008) 69–74
Abstract: Alanine racemase activity was investigated in Bacillus subtilis. A putative second alanine racemase gene (yncD) was cloned in parallel with the previously identified alanine racemase gene, dal. Each of the B. subtilis genes, dal and yncD complemented the Escherichia coli Alr DadX double mutant alanine auxotrophic strain MB2159 in vivo, restoring the prototrophic phenotype. Alanine racemase activity was also detected in vitro in cell-free extracts prepared from cultures of E. coli MB2159 harboring plasmids expressing either of the cloned B. subtilis genes and preliminary characterization of enzyme activity is presented.
Description: An article published by FEMS Microbiol Lett, 283 (2008) 69–74; DOI:10.1111/j.1574-6968.2008.01151.x
URI: http://hdl.handle.net/123456789/11155
Appears in Collections:College of Science

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