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|Title: ||Isolation and characterization of soilborne virulent bacteriophages infecting the pathogen Rhodococcus equi|
|Authors: ||Salifu, Samson Pandam|
Casey, S. A. Campbell
|Issue Date: ||2013|
|Publisher: ||Journal of Applied Microbiology|
|Citation: ||Journal of Applied Microbiology, 114,1625--1633, doi:10.1111/jam.12194|
|Abstract: ||Aims: To isolate and characterize a diversity of bacteriophages (phages) that
infect the soilborne pathogen Rhodococcus equi.
Methods and Results: Twenty-seven phages were isolated from soil samples
from geographically distinct locations using a range of R. equi bacterial strains,
including clinical isolates. On the basis of host range, genomic DNA restriction
profiles and virion protein profiles, the diversity of these phages was extensive,
with phages being divided into 16 groupings.
Conclusions: Based on a range of criteria, these phages could be divided into
16 distinct groupings. The majority of the phages recovered from soil were
Siphoviridae, adding to the limited number of Siphoviridae described to date
for R. equi. One grouping consisted of phages belonging to the Myoviridae.
Significance and Impact of the Study: This represents the first study looking
at the diversity of phages infecting the pathogen R. equi, including the first
Myoviridae to be isolated and characterized for the genus Rhodococcus and for
the nonmycobacterial actinomycetes. Given their diverse host range, including
clinical isolates, this collection of phages offers the potential for the
development of phage cocktails for use as a therapeutic or alternatively in the
biocontrol of this pathogen in reservoirs of infection relating to animal
|Description: ||An article published by Journal of Applied Microbiology, 114,1625-1633, doi:10.1111/jam.12194|
|Appears in Collections:||College of Science|
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