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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11150

Title: Genome and proteome analysis of phage E3 infecting the soil-borne actinomycete Rhodococcus equi
Authors: Salifu, Samson Pandam
Valero-Rello, Ana
Campbell, Samantha A.
Inglis, Neil F.
Scortti, Mariela
Foley, Sophie
Vázquez-Boland, José A.
Issue Date: 2013
Publisher: Environmental Microbiology Reports
Citation: Environmental Microbiology Reports (2013) 5(1), 170–178, doi:10.1111/1758-2229.12028
Abstract: We report on the characterization and genomic analysis of bacteriophage E3 isolated from soil and propagating in Rhodococcus equi strains. Phage E3 has a circular genome of 142 563 bp and is the first Myoviridae reported for the genus Rhodococcus and for a non-mycobacterial actinomycete. Phylogenetic analyses placed E3 in a distinct Myoviridae clade together with Mycobacterium phages Bxz1 and Myrna. The highly syntenic genomes of this myoviridal group comprise vertically evolving core phage modules flanked by hyperplastic regions specific to each phage and rich in horizontally acquired DNA. The hyperplastic regions contain numerous tRNA genes in the mycobacteriophages which are absent in E3, possibly reflecting bacterial host-specific translation-related phage fitness constraints associated with rate-limiting tRNAs.Astructural proteome analysis identified 28 E3 polypeptides, including 15 not previously known to be virion-associated proteins. The E3 genome and comparative analysis provide insight into short-term genome evolution and adaptive plasticity in tailed phages from the environmental microbiome.
Description: An article published by Environmental Microbiology Reports (2013) 5(1), 170–178, doi:10.1111/1758-2229.12028
URI: http://hdl.handle.net/123456789/11150
Appears in Collections:College of Science

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