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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11065

Title: Molecular Characterization of Cocoa, Mango, Banana and Yam Isolates of Botryodiplodia Theobromae in Ghana
Authors: Twumasi, Peter
Moses, E.
Ohene-Mensah, G.
Issue Date: 2013
Publisher: Journal of Ghana Science Association
Citation: Journal of Ghana Science Association, Vol. 15 No. 1, 2013
Abstract: Botryodiplodia theobromae is a virulent plant pathogen commonly found in the tropics and subtropics. The fungus has wide range of plant hosts and known to cause yield losses up to 80% especially on cash and food crop farms. This study aimed at establishing genetic diversity of B. theobromae collected from four common food and cash crops grown in Ghana. A total of 25 fungal isolates were sampled from cocoa, mango, banana and yam within four geographical regions of Ghana. The isolates were developed into pure single-spore cultures on Potato Dextrose Agar (PDA). Single-spore cultures of the 25 B. theobromae isolates from the four crops were grown in V8 juice medium at 28°C on a rotary shaker for 48 hrs. Mycelia were harvested at 48 hrs of growth, washed with sterile distilled water, grounded in liquid nitrogen and the genomic DNA isolated. PCR products from SSR and RAPD primers were resolved on 2.5% agarose gel and the DNA bands from the 25 isolates clustered on dendrogram into 5 distinct groups of varied genetic similarities using NTSYS software. The study showed only two banana isolates, B(Kt) and B(Ef), sharing highest genetic similarity above 80%. An isolate from yam, Y(Zu), shared no genetic similarity (0%) with any of the remaining 24 fungal isolates from the four regions in Ghana. The remaining 22 isolates measured genetic similarity between 20 and 75%. The results suggest high genetic variability among the B. theobromae isolates on the crops studied.
Description: An article published by Journal of Ghana Science Association, Vol. 15 No. 1, 2013
URI: http://hdl.handle.net/123456789/11065
Appears in Collections:College of Science

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