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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/10687

Title: Determination of the Rutin, Quercetin and Kaempferol Contents in a Ghanaian Polyherbal Formulation (EAF-2011) and Its Raw Materials Using a Simultaneous RP-HPLC Method
Authors: Thomford, K. P.
Mensah, M. L. K.
Dickson, R. A.
Sakyiamah, M.
Edoh, E. A.
Annan, K.
Keywords: Herbal medicines
Polyherbal product
Quality Control
RP-HPLC
Standardisation
Issue Date: 2015
Publisher: International Journal of Pharmaceutical Sciences and Drug Research
Citation: International Journal of Pharmaceutical Sciences and Drug Research 2015; 7(1): 96-99
Abstract: Quantitative chemical fingerprinting of herbal medicines provides an avenue for addressing the challenge with their quality. In this report, chemical markers that could be labelled as active markers in the polyherbal product and its component raw materials were assayed using a simultaneous RP-HPLC method. These markers were rutin (RU), quercetin (QE) and kaempferol (KA) which are well known for their antimicrobial activities. The quantitative assay of the markers using a validated HPLC method showed the herbal product (EAF-2011) contained 8.6810% (w/w) of rutin, 0.2670% (w/w) of quercetin and 0.0610% (w/w) of kaempferol. At least two (2) of these flavonoids were detected in each of the component raw plant materials: Psidium guajava [(RU-8.5860% (w/w); QE-0.5430% (w/w); KA - 0.1003% (w/w)], Alchornea cordifolia [RU-2.5540% (w/w); QE-0.0536% (w/w)], Zanthoxylum zanthoxyloides [RU-5.8060 % (w/w); KA-0.0174% (w/w)], Tridax procumbens [RU-2.4390% (w/w); QE-0.1040% (w/w); KA-0.0510% (w/w)] and Eugenia caryophyllata [QE-0.4080% (w/w); KA-0.0771% (w/w)]. The results obtained indicate that the HPLC method, selected chemical markers and chromatograms produced are ideal for the quality control of the product and its component raw materials.
Description: An article published by International Journal of Pharmaceutical Sciences and Drug Research 2015; 7(1): 96-99
URI: http://hdl.handle.net/123456789/10687
Appears in Collections:College of Health Sciences

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